PKTUARTTest error

Is this error raised by a firmware downloaded from Mesa?

no, i took both of them from here:
github.com/LinuxCNC/hostmot2-firmware/bl...N_PktUARTTest_72.vhd
then i add it into xilinx library

PktUARTTest_72 is for a 72-pin board.
You have a 34-pin board (5i25) I think, so you need to use a 34 IO pin firmware:
github.com/LinuxCNC/hostmot2-firmware/bl..._PktUART1inst_34.vhd

Right, you need to use a pinout file (or create one) that has the correct number of pins
and the correct number of I/O ports (34 pins and 2 ports for a 5I25)

The actual driver error message is caused by using a pinout file for a device that has 3 I/O ports,
not 2 I/O ports like a 5I25